408

TABLE 13.2 (Continued)

Plant Species

Organ Used for

Fractionation

Experimental

Condition

Technique

Used

No. of

Identified

DAP (Differentially Abundant Proteins)

References

Proteins

Oryza sativa

Root tip

Salt

2DE-IEF/

SDS-PAGE

8

Up: Glycoside hydrolase, Cu/Zn-SOD, 20S

proteasome subunit, HSP70.

Chen et al.

(2009)

Down: cytochrome-c oxidase subunit 6b, ATP

synthase beta subunit S-adenosylmethionine

synthetase 2, transcription initiation factor eIF-3

epsilon

Triticum aestivum

Wyalkatchem (T),

Calingiri, Janz (S)

Shoots

200 mM NaCl

Isolation:

PVP gradient;

acetone

extraction;

2D-DIGE

LC-MS/MS

192

Up: Mn-SOD, AOX, VDAC

Down: CS, NDPK, outer mitochondrial

membrane porin

Genotypic differences: AOX, Mn-SOD –

increased in T

Jacoby et al.

(2010)

Glycine max

Root and

hypocotyl

Flooding

2-DE/BN­

PAGE/MALDI

TOF MS

52

Up: NADH-ubiquinone oxidoreductase, TCA

cycle enzymes; GroES chaperonin, EF-Tu, porin,

VDAC.

Komatsu et

al. (2011)

Down: cytochrome c, Tim, Tom20; protein

components of complexes III, IV, and V of ETC

Pisum sativum

Leaves

150 mM NaCl

2-DE/MALDI

TOF MS

–

SNO modified proteins: ATP synthase β, HSP90,

SHMT; Prx IIF activity were decreased

Camejo et al.

(2013)

Triticum aestivum

Chinese Spring

(S), T. aestivum

× Lophopyrum

elongatum

amphiploid (T)

Shoot and root

200 mM NaCl

100% acetone

(leaf), TCA/

acetone (root);

2D-DIGE

MALDI-TOF/

TOF; HPLC

Q-TOF MS/MS

68

Genotypic differences: Mn-SOD, MDH,

aconitase, SHMT, β-CAS – increased in T; Up in

shoot-aspartate aminotransferase, GDH

Jacoby et al.

(2013)

Biology and Biotechnology of Environmental Stress Tolerance in Plants, Volume 3